Duo Spore
Duo-Spore® Biological Indicator Test With Culture Service Program features Biological Indicator Strips prepared from suspensions of Geobacillus stearothermophilus and Bacillus atrophaeus, encased in a peel-open glassine pouch. The glassine pouch allows for exposure of the enclosed strip to the sterilant without the risk of contamination, allowing for worry-free transfer to culture media after cycle completion.
What is the Propper Biological Indicator Culture Service Program?
Utilizing the Culture Service Program can help you avoid incubating indicator strips at your facility. Simply mail your processed indicators to the Propper Laboratory for this convenient, done-for-you service. Biological indicator results are then provided within a 48-hour incubation period and are accessible through our secure online portal. Each test envelope conveniently contains two test strips and one control strip, separated by color-coded pouches for ease and accuracy in biological testing.
Features and Benefits of Propper Culture Service
Compatible with dry heat, chemical, ethylene oxide (EO), and steam sterilizers.
Quick results upon receipt of spore strips in our laboratory by email and on our secure, confidential web portal.
Failed tests are notified by phone call from our laboratory team in addition to email notification.
Highly resistant dual-species bacterial spores provide the most reliable method of testing for an effective sterilization cycle.
Use of Duo-Spore® Biological Indicator
AAMI and the Joint Commission on Accreditation of Health Care Organizations, facilities must perform biological monitoring.:
Daily with ethylene oxide sterilizers
At least weekly and preferably daily with steam and dry heat sterilizers
NOTE: Monitor every load containing implants with biological indicators.
Important Update for 2024: Our Duo-Spore® Biological Indicators will now feature white translucent glassine paper instead of blue. This change is thoroughly tested and doesn’t affect functionality. The transition will happen in the coming months and will have no impact on your processes, delivery, or storage conditions.

