Duo Spore Bio Indicator
Duo-Spore® Biological Indicator Test are reliable for monitoring steam, ethylene oxide, or dry heat sterilization cycles. These strips are prepared from Geobacillus stearothermophilus and Bacillus atrophaeus and come with two test strips and one control strip in two color-separated pouches.
To use these strips, process them using Tryptic Soy Broth (TSB) culture media in your facility. Propper Tryptic Soy Broth features a higher spore recovery rate, ensuring a more dependable test.
Positive test results may vary based on the media and source, most occurring within 24 hours for steam and 48 hours for ethylene oxide. In some cases, sub-lethally damaged spores may require longer incubation periods, up to 5 days.
Additionally, D-values and Survival/Kill performance data supplied with each unit comply with U.S. Pharmacopeia requirements. For reliable sterilization cycle monitoring, choose Duo Spore® Biological Indicator Strips.
Additionally, the Duo-Spore® Biological Indicator Test With Culture Service provides a reliable and compliant method to ensure effective sterilization cycles in clinical and non-clinical settings. This saves time and effort with Propper Manufacturing’s convenient Mail-in Culture Service Program. Furthermore, our online portal allows for easy access to your results.
Important Update for 2024: Our Duo-Spore® Biological Indicators will now feature white translucent glassine paper instead of blue. This change is thoroughly tested and doesn’t affect functionality. The transition will happen in the coming months and will have no impact on your processes, delivery, or storage conditions.

